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MTT assay was used to assess cell viability, triglyceride quantification and oil red O staining were used to determine intracellular lipids content whilst ketone bodies were measuredAdd Oil Red O working solution for 10 min (do not touch walls of the wells) Remove all Oil Red O and IMMEDIATELY adddH2O, wash with H2O 4 times (you can wash under running tap water)
Oil red staining protocol cell culture
Oil red staining protocol cell culture-Bovine adipofibroblasts, 3T3L1 cells, L6 myogenic cells, and sheep satellite cells were allowed to proliferate for 48 h Oil redO (ORO) was dissolved in three different solvents isopropanol,For 1 year To make Oil Red O Working Solution, add 3 parts of Oil Red O Stock Solution to 2 parts of dH 2 O, mix well, and allow to sit for 10 min Filter with 02 µm syringe filter or Whatman No 1
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Oil Red O Staining for Cultured Cells Culture and treat cultured cells in tissue culture plate as needed (see other protocols) Take the plate (35mm) out of incubator and remove the mediumOil RedO Staining of Adipocytes Prepare Oil RedO Stock is 05% in isopropanol To make working solution, add 60 mL stock to 40 mL H2O (or anything with that ratio), remove precipitatePreparing oil red o stain • Prepare the stock solution by weighing out 300 mg of oil red o powder and adding this to 100 ml of 99% isopropanol This solution is stable for one year from the date
The Lipid (Oil Red O) Staining Kit is suitable for selective staining and detection of neutral lipids in cultured cells Hematoxylin included in the kit stains the nuclei of the cells Components The kitPreparation of Oil Red O staining solution Oil RedO Stock Solution • Oil RedO 030 g (300 mg) • 2propanol, 99% 100 ml Mix well Transfer to a bottle and cap tightly Store at room temperatureOil Red O stains lipid vesicles which are created by functional adipocytes and is a useful tool when used with Lifeline ® human mesenchymal stem cells and Lifeline ® AdipoLife TM DfKt TM 1 or
Oil red staining protocol cell cultureのギャラリー
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 Human White Preadipocytes Hwp Promocell | Human White Preadipocytes Hwp Promocell |  Human White Preadipocytes Hwp Promocell |
 Human White Preadipocytes Hwp Promocell | Human White Preadipocytes Hwp Promocell |  Human White Preadipocytes Hwp Promocell |
 Human White Preadipocytes Hwp Promocell |  Human White Preadipocytes Hwp Promocell |  Human White Preadipocytes Hwp Promocell |
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